FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443069
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9200.017.6248591
CTATACT10250.015.1462214
GTATATG12650.014.18170451
TATAGTG10000.014.1448865
TATTAGG9800.013.7294832
TCCTATA8050.013.7141752
TATACTG14250.013.5577865
TCTATAC10450.013.5357783
CCTAGAC4609.094947E-1213.4998923
CCTATAC8300.012.8854393
CCGTGTA2457.732153E-512.6731099
GTATAGA13000.012.4729781
TCTACAC10250.012.453563
TAGGGGT3902.7326678E-812.3845174
CTAGACC6200.012.2418374
GTATAGG7900.012.2277511
TATAGGG6500.012.2075952
CTGTGCA6250.012.1436559
ATAGGGG4853.110472E-1012.0926873
TATATAC20300.012.0664053
Produced by FastQC (version 0.11.2)