FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499131
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGC200.007357874451.74774655
TCGGGGG450.004618931630.66840616
CGGGGGC500.00772865827.60156217
CGGTCGG500.00772865827.60156213
GCAGCGT1850.026.1069721
GCGTCAG1850.026.1069724
CAGCGTC1901.8189894E-1225.4199452
CCGCACC951.0495698E-525.41994565
AGCGTCA2103.6379788E-1222.9989993
CTGGGCA1052.2782018E-522.9989999
CGTCAGA2103.6379788E-1222.9989995
TCTATAC1854.820322E-1022.3774033
TCCGCAC800.002897891721.5615664
GAGACAG2501.8189894E-1220.69909921
CTGTGTG850.00411800820.2932349
CAGATGT2354.5838533E-1019.0842748
GATGTGT2406.0572347E-1018.68668610
ACCGTTG1301.1797216E-418.5761138
CCATGAG950.00783037118.1571039
CGCAAGG950.00783037118.15710369
Produced by FastQC (version 0.11.2)