FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934922
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8200.020.1949481
GACCTCG1852.6463204E-718.6480067
TACACAG6100.018.0982045
ACCTCGC1954.5987326E-717.6916988
TATTAGG9050.016.0109122
TATACTG11950.015.8785255
GTCTATA5900.015.7879991
TCTATAC8600.015.6452133
TAGGACA5750.015.5998654
TATACAG13000.015.3921765
GTGTAAG3959.094947E-1214.8479741
CCTAATA8950.014.6479182
ACCGTGC3302.6229827E-914.635868
CGCACCT3808.367351E-1114.5254466
TAGGTAG5000.014.4898755
CGCGCGC1450.005215995514.27610823
TCTAGGG2901.2608507E-714.2757392
ACTATGC8700.014.2753728
TCCGCAC3901.2732926E-1014.15299364
TGTGCGA3202.7597707E-814.01477910
Produced by FastQC (version 0.11.2)