FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557251
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4550.021.9884055
TAGTCAG3100.018.9188379
CTAATAC7000.018.7280563
TATTAGG5350.018.055582
GGTAGTC4600.017.9995087
CCTAATA6600.017.772242
GTATAGA7500.017.0195331
GTATTAC6100.016.401191
GTATTAG6850.016.1163461
GTATTAT9900.016.0298611
TTAGGTA5500.015.6813874
TATACAG8550.015.3329125
TAATACT8850.015.2029724
TATTATA9700.014.9377342
GTATATA10050.014.7607891
TATACTG9600.014.7339715
TATATAC11400.014.2232933
CCTACAC3202.7572241E-814.015243
TCCGCAC3253.3798642E-813.79962164
TATAGAA9800.013.7292152
Produced by FastQC (version 0.11.2)