FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633549
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG4350.017.4480671
GTATTAG5000.017.2497921
TAGGTAG4050.017.0368335
GTCCTAA3451.8189894E-1216.9997961
TAGGGGG1452.7235455E-416.6549744
TATACTG8400.015.6069565
TCCTAAC4000.015.5248142
CTAGGAC2503.672103E-715.1798173
TCTAGGA4700.014.6806762
CTTACAC3808.367351E-1114.5261413
GTATAAA7500.014.25982951
ATAGGGG3152.2415406E-814.2379253
GCATAGG2202.8124054E-514.1134671
CTAGGAT3708.731149E-1013.9863193
TATAGGG4455.456968E-1213.9548892
GTCCTAC2001.9824642E-413.7998341
TATCCCG2751.0782369E-613.7998345
GTATAAG5350.013.5418941
TGTATAC7150.013.5103283
CCTACAC2851.6117519E-613.315633
Produced by FastQC (version 0.11.2)