FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617523
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT1750.037.4568371
GCGTCAG1800.036.419324
CTGAGCG400.002595512734.4997189
CAGCGTC2600.025.2133732
AGCGTCA2650.024.7376483
CGTCAGA2650.024.7376485
CCGCACC1154.5984943E-520.99982865
GATGTGT3100.020.03209310
GAGACAG2950.019.88119321
GGCGGTG1056.0189934E-419.71412531
TAGGTAG1258.727798E-519.3214055
TTTCCGC2053.8144208E-818.51204362
TCCGCAC1501.6919694E-518.39984964
TATACTG4900.018.3074555
TGAGTCA1552.2434648E-517.80630510
AGATGTG3700.017.716079
GTCAGAT3750.017.4812726
ACAGTGC1200.001475011817.2512558
ACTGAGC1200.001475011817.2512558
GTATTAG3003.6379788E-1117.2498591
Produced by FastQC (version 0.11.2)