FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706963
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG5100.014.8821455
TAGACTG5550.013.6754865
ACTATGC10700.013.2191978
TAGGTAG6550.013.1677565
CGCACCT5103.6379788E-1212.85228766
CTAATAC12800.012.6677923
TATACGG8150.012.2759025
TACTATG12150.012.209487
TAATACT19750.011.8783154
CCGACCC2357.7299046E-411.74495144
TACAGCG5905.456968E-1211.6945367
TCCGCAC6201.8189894E-1211.68488964
GTATTAG10700.011.6077431
CAGACCG2701.9141029E-411.4994159
ACAGTCC5451.7826096E-1011.3941268
TATACTG12800.011.3201545
GTACAGG5804.3655746E-1111.3019841
CCTAATA12850.011.2763172
GTATAAG12050.011.1662321
GACGGAC18850.011.16388869
Produced by FastQC (version 0.11.2)