FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131333
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG2901.8189894E-1219.0344055
CCTAATA6650.016.6014372
CTAGGAC2552.6018824E-816.2352283
CCTAGCG4150.015.79696930
GTATAGA9800.015.4897321
GTATTAG6350.015.2125371
CTAATAC8400.014.7856543
TTATACT7800.014.5960944
TAGACTG3554.6929927E-1014.5774065
GTATAAG7150.014.4754671
TACTAGA8350.014.4610192
CGATCAC5250.014.45708424
TAATACT10850.014.3086984
TGGACCG4101.8189894E-1114.304825
GACCGCG3202.759225E-814.0152397
ACCCGCG3253.3758624E-813.80156233
TAGAACC7850.013.6241484
ATTATAC9200.013.4999463
GTATAAA10250.013.463361
TATACCG6750.013.2888355
Produced by FastQC (version 0.11.2)