FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174859
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC4950.018.8185353
CCTAATA7350.014.5512922
GTATAGA11700.014.1543341
TATACAG11050.014.0500375
TAGGACA7150.013.9932684
CTAGACT4304.1836756E-1113.639794
CTATACT9700.013.5157174
ACCTAGG4153.4197E-1013.3016621
CTAATAC9000.013.0335773
TCTATAC10700.012.8974373
GTATAGG10300.012.7285941
GGCTAGG3551.0736221E-712.6342371
TAGGGAG5105.638867E-1112.1766995
GTATTAG9150.012.06598951
TAGACTG6600.012.0229525
TATATAC12550.011.8209383
GTATATA10950.011.6579361
GTATAAG9200.011.6254011
GTGTAAG7750.011.5745941
TCTAGTA10900.011.3947092
Produced by FastQC (version 0.11.2)