FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634747
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG2050.028.6136114
GCAGCGT2150.027.2805961
CAGCGTC2550.023.00312
AGCGTCA2700.021.725153
GTGTAGG900.005729796419.1677381
GTATTAG3250.019.1087631
CGTCAGA3100.018.9219045
CTAATAC3200.018.3305953
TAGTGGG1351.5748556E-417.889897
GTAGACA2005.969505E-717.2523254
GATGTGT3451.8189894E-1216.99961310
TAGGTAG1855.4322136E-616.7860455
GTCAGAT3501.8189894E-1216.7594016
GTATAGA3309.094947E-1216.7282091
TCAGGAC1250.001937575916.5622313
TAGGACT1250.001937575916.5622314
ACACCGT2301.417593E-716.5022226
GTGTAAG1907.0053266E-616.3430181
ACAGGCA1958.964909E-615.9239678
TCCGCAC1300.002520426615.92271364
Produced by FastQC (version 0.11.2)