FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261012
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC3050.023.7529493
GTCTTAG2350.022.0206991
CCTAATA2650.020.829182
TAATACT4100.020.1941454
GTATTAG2707.2759576E-1219.1661621
GTATAGA4750.018.1574171
CCTTATA3051.8189894E-1218.0974852
CCTTAAG1552.2481045E-517.8059841
CCCTAAG1402.0841183E-417.2495461
GTCCTAC2251.1092743E-716.8662241
ACCTAAT3309.094947E-1216.7268331
TATTAGG2702.926754E-916.6103062
GTGTAGG3601.8189894E-1216.2912391
GTATACG5750.016.1995741
TATAGGG3006.82121E-1016.099222
TTATACG2151.2771488E-616.0457344
TAGACAG2804.7402864E-916.0170825
TAGAACT4750.015.9781734
GTTATAC2402.3127723E-715.8117353
TTAGGCT1554.5281934E-415.5798914
Produced by FastQC (version 0.11.2)