FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948786
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT4350.019.8274384
GTATAGA5400.018.527641
GTATAGG5850.018.2819161
TATAGGA3650.017.958772
TCTATAC4700.016.8828543
CTCCTAT6300.015.88083569
TATACTT5000.015.8698815
ATAGGAT3058.60382E-1015.8359483
ATATAGT5350.015.4765211
TACACCG5350.015.4765215
TAGACTG3151.3587851E-915.333225
TATACCG4850.014.9380325
TTACACG2554.5966044E-714.8822414
CACTAGA4650.014.83862
ACTAGAC2808.2069164E-814.7856043
TATTATA5400.014.6943352
CCTAGCG2353.2171338E-614.6822530
TAAGCAG7850.014.50307755
TATAGTA3605.7843863E-1014.3748932
TATACGG4101.8189894E-1114.3047725
Produced by FastQC (version 0.11.2)