FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542836
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4950.018.1211645
CTAGGGG1954.5986053E-717.6922633
TATACAG11400.016.9473275
GTATATA14150.015.6048131
TAGACTG4600.014.9999625
CTAATAC10150.014.9556273
GTGTAAG5650.014.6554061
GTATTAG8650.014.358911
TATTATA14350.014.185192
ACAGTGC7100.014.0915148
GTAGGAC4202.7284841E-1113.9642513
GTATAAA11700.013.8594841
TATACTG13200.013.8522375
TAATACT14000.013.7999644
CCTAATA9300.013.7263112
TAGATAG8050.013.71425155
GTATAGA10200.013.5299091
ATAGGAC6000.013.2249663
TTATACT12050.013.170094
TACACCG5250.013.1428235
Produced by FastQC (version 0.11.2)