FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332908
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG200.0073549151.7492144
GCGTCTT500.007727730627.5995861
TTAGGGA750.001990242922.9996514
TCTACAC1253.4913228E-622.0796643
TATACAG1756.162736E-921.6853855
GTCATAG800.002895410621.5621721
TCTATAC1304.905147E-621.2304483
CCTATGT850.004114499320.2938083
CTAATAC1056.0112885E-419.7139853
ACTGCTC900.005726547419.1663769
TAATACC2105.005859E-818.0711544
GTATATA4900.016.8977011
CTAGATA1554.5126295E-415.5804084
TACACCA1554.5126295E-415.5804085
TTATACT2907.4614945E-915.4652814
GTATAGA1851.0179842E-414.9186921
CCTTGGA1400.00412306514.785492
TAAGATA2101.8046023E-514.7854894
TCTTGAC1657.25321E-414.636142
GGTCAAG1450.005205120414.2756431
Produced by FastQC (version 0.11.2)