FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094475
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG2907.545168E-915.46482913
TAGGCCG1901.2830485E-414.5259055
ACCGTGC3557.3396222E-913.604818
CTAATAC13700.013.0945213
TAGGGGG3255.054062E-712.73814
TCGGGGG3002.8693958E-612.64943816
TAGGACT5800.012.4910254
GTCGGGG3104.1411095E-612.24139115
AACCGTG5602.3646862E-1111.7046477
CACGAGC6201.8189894E-1211.68477569
GTATATA21150.011.4186711
GAGACTC47050.011.36487969
GAGCGAC3652.0540865E-611.34177911
ACTCCTG32600.011.21711269
GTATACG8000.011.2127271
ATAGGAC6800.011.16144853
CTAGACA7750.011.1287174
CTAGGGG2500.001298263811.0396873
AGACTCC40700.010.93422669
TATACCG8000.010.7809455
Produced by FastQC (version 0.11.2)