FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805708
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4150.019.9513591
GTATAGA7200.016.7704561
ATATACT7700.016.5775494
TATAGGG4500.016.0996382
TATACAG7100.015.5489465
TATAGAG6450.015.5112782
GTCTATA4700.015.4145481
CTAGACT3603.45608E-1115.3329894
TATTATA6300.015.3329892
TATACTG9050.015.2482785
GTCTAGG7100.015.0630421
GTCCTAA3907.2759576E-1215.0381221
TCTAGAC6200.015.0238553
TATTAGG3705.4569682E-1114.9185842
TATATAC7650.014.4310483
ATTATAC6600.014.1133193
GGTTGCG5150.014.06803547
GGCCATA6200.013.91097753
TCTATAC6500.013.799693
TCTAAGC6050.013.6856433
Produced by FastQC (version 0.11.2)