FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998655
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT5900.022.80413666
GTATTAG13350.021.1908361
TAGGTAG6950.020.8487515
TCCGCAC6700.019.5663564
CCGCACC7500.017.93925565
CTAATAC15450.016.7474353
TATTAGG13300.016.0828422
TCTATAC14350.015.6270483
CCTAATA16150.015.3809352
ATTAGGT13100.015.2746833
ACTATGC14250.015.2522538
TAGTCAG7900.014.8474849
TTAGGTA11650.014.8067454
GTATAGA15400.014.7855931
TAATACT23950.014.2608424
TCACACA10950.014.17891130
CTATGCA12900.014.173839
GGTAGTC9300.013.7254657
GTATATA23200.013.68092251
TATACCG9100.013.648245
Produced by FastQC (version 0.11.2)