FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930518
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3450.022.9999735
GTATTAG6400.021.0234131
TTAGGTA4600.019.4999794
TATTAGG5000.019.3199772
TATACAG7000.019.2214075
TAGTCAG3300.018.8176759
GTATAGA7500.017.479981
ATTAGGT5400.017.249983
TATACCG5850.017.1025455
CTAATAC4750.016.7052443
GTATATA9300.016.6935311
GTCTATA4200.016.4285531
CCTAATA4650.016.3225632
ACCTAAT4550.015.9230591
ATAGGAG4150.015.79598133
TAGACTG3552.910383E-1115.5492785
CCTAGCG2452.9189323E-715.4905830
ACATAGG4500.015.3341131
TAGACCG4500.015.3333165
GTATAAT6750.015.3333161
Produced by FastQC (version 0.11.2)