FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462193
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGC450.00462092530.6652248
GACCCGT450.00462092530.66522417
CAGTCCG450.00462092530.6652249
GCGTCAG1356.8115914E-620.4434834
CGTCAGA1356.8115914E-620.4434835
CTACCCT1056.017427E-419.7133584
ATGTGTA1751.4687066E-719.71335611
ACCGGCG900.00572731619.1678430
GTATATG2901.8189894E-1219.0335871
GATAGGT950.00782967418.157047
TCAGATG1602.9453655E-517.249197
TAGGTAG1402.0799325E-417.2491875
GTCCATA1200.00147543517.2491871
TATACCG1452.7189736E-416.6543885
GTCTATA1250.001938856716.5592211
CCTATAC1250.001938856716.5592213
TAAGACA1907.003904E-616.3413374
TTAAGAC1503.5206918E-416.0992433
CTTAAGA1958.963112E-615.9223292
CTCTTAG1300.00251944315.9223281
Produced by FastQC (version 0.11.2)