FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059596
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC12050.016.6052468
GTATAGA13400.016.4778611
GTATATA17450.016.409991
GTATTAG8150.015.2394951
TCTATAC10250.014.8094973
TATACTG15700.014.0634475
GTATAGG9150.013.9510311
ATACCGT11900.013.9154966
GTATATG12100.013.6861581
TCCTAGG3801.3042154E-913.6184052
TATACAG13950.013.60191255
TAATACT15550.013.5335254
GTATAAG10750.013.1583391
ACCTAAT11100.013.0542531
TATACCG9050.012.9610985
GTATAAA13850.012.9532651
CCCTATA6200.012.7983722
TATATAC17950.012.6850153
TACACCG6800.012.68365
CTAATAC10900.012.6603293
Produced by FastQC (version 0.11.2)