FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410065
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1101.0531949E-931.3651334
CTAGGAC802.7549122E-630.188943
CAGCGTC1254.132744E-927.6013182
CGTCGCG500.00773201327.59795210
ACGCACT809.544034E-525.8762344
GACTGTG809.5519514E-525.873087
GCAGCGT1359.384166E-925.5567761
ACTGTGC851.4418169E-424.3511338
TCTAGAC750.001990296923.0010993
TAGGACT902.1224275E-423.0010994
AGCGTCA1657.881317E-820.910093
AGACAGG1004.330426E-420.69846322
CTACACT1206.3719286E-520.125964
CATATAC3400.019.2803333
CGTCAGA1903.4821642E-718.1587625
ACTGTGT1351.5747052E-417.8875628
GAGACAG2005.9698505E-717.2487221
GTCAGAT2251.10183464E-716.8674726
GATGTGT2057.7301047E-716.8280210
CAGATGT2057.7301047E-716.828028
Produced by FastQC (version 0.11.2)