FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212249
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG10550.022.2363969
GCCGTCT12250.021.12255946
CGTATGC13250.020.30985641
GTATGCC13600.020.04085742
TATGCCG13600.019.78717443
TGCCGTC13750.019.5710145
ATGCCGT13750.019.320144
TCGTATG14650.018.36898240
CTCGTAT14500.017.36932439
ATCTCGT14800.016.785737
CCGTCTT16900.015.9231647
AAAGGGG15150.015.2570368
TCTCGTA16250.015.07556638
AATCTCG17250.014.00159836
GACAGGC78650.013.90491769
CTGCTTG20850.013.7336754
CACGAGC5600.013.55321969
TAATACT17600.013.3289924
CTAATAC11950.013.2797833
GTATAGA11700.013.26931
Produced by FastQC (version 0.11.2)