FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5213088
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19900.016.2971271
TATTAGG20000.015.5250182
TATACTG28400.015.3060625
TAGGTAG11550.014.9347955
TCCGCAC9650.013.9426264
CGCACCT9850.013.65965266
TATACCG14200.013.6053885
TAATACT34300.013.477894
GTATAGA22100.013.4258681
GTATAGG17250.013.0005131
TTATACT30300.012.9799634
TAGGACA12350.012.8499714
ACCGCGC5205.456968E-1212.605428
CCTAATA24400.012.584032
GTCCTAA12350.012.2919841
GTATATA33750.012.0626981
TCTATAC21500.012.0346663
ACAGTGC14100.011.989038
CTAATAC21650.011.9512853
CCGCACC11300.011.90675165
Produced by FastQC (version 0.11.2)