FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4219555
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT22500.015.3328654
CCTAATA14850.015.1009052
GTATAGG13000.014.5962271
CTATACT13950.014.0963434
GTATAGA17550.013.95733451
TACACAG12400.013.9108655
CTAATAC17200.013.8396933
GACAGGC38050.013.60027169
GTATTAG14750.013.332271
CTATGCA13350.012.92089
ACTATGC17600.012.5449228
TATTAGG12950.012.521152
TACTATG16650.012.4319067
TAGGTAG7250.012.3720355
ATACTAT21550.012.326776
GTATATA21050.011.8005331
TATATAC24850.011.8004443
CGCACCT8550.011.70139666
TCCGCAC8800.011.36883564
ACCTAAT18000.011.308391
Produced by FastQC (version 0.11.2)