FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875916
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC4150.035.745542
GCAGCGT4400.033.7145421
GCGTCAG4400.033.7145424
AGCGTCA4800.030.9049973
GCGGGGT450.004623013530.66542213
CGTCAGA5550.026.7286455
GATGTGT5750.025.79895410
ATGTGTA6650.023.86369511
GTGTATA6350.023.3612613
TGTGTAT6800.022.82995812
GTATAAG6400.022.63970815
CAGATGT6600.022.4763628
AGATGTG6650.022.3073679
TCAGATG7250.020.4612397
CCGCACC1356.822682E-620.44361565
GTCAGAT7400.020.0464866
ATAAGAG7750.019.58630417
AGAGACA7800.019.4607520
TCCGCAC1301.18098644E-418.5761764
GAGACAG8300.018.28841621
Produced by FastQC (version 0.11.2)