FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141058
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC9250.017.5286853
TATACTG11350.016.413125
TAGGTAG4500.015.3324195
GTATTAG8600.014.8431791
CGCACCT5600.014.78483366
TCCGCAC5500.014.42641464
ACTCCGC4202.7284841E-1113.9634538
TATACCG5450.013.9257765
ACCGTGC4304.1836756E-1113.6387218
CTATACT7850.013.6233924
CTAGGAC3204.1867497E-712.9367293
TATATAC19250.012.9031283
TACTATG10450.012.8748317
AGAGTGT8350.012.807626
GTATAAA15100.012.7948361
TTATACT12500.012.6952444
TATTAGG8450.012.656052
GTATATA17750.012.6339351
ATACTAT14500.012.6095946
CTAGGGC2509.341764E-512.419263
Produced by FastQC (version 0.11.2)