FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005204
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA14950.019.1547051
GTATTAG11300.017.4034351
CTAATAC13850.016.4398043
CCTAATA14650.016.0133042
TAGGTAG7550.015.9927645
TATACTG21100.015.5325795
CCTAGCG4750.015.25483830
ACTATGC12250.015.207588
TCTATAC13200.014.8971553
CTATGCA11250.014.4127819
TCCGCAC7500.014.25945364
GTCTATA8400.013.9649111
TATACAG17550.013.956735
TACTATG13950.013.601637
CTAGGCA6100.013.5732510
GTACTAG8150.013.5466191
GACTAGG5450.013.2930698
AGACTAG6000.013.2244937
TATAGAG16750.013.1818042
CGCACCT8700.013.08592366
Produced by FastQC (version 0.11.2)