FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103465
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13500.019.1668151
TAGGTAG7950.016.9246585
TATTAGG12900.015.2443042
TAGTCAG6400.015.093389
CTATACT14000.015.032264
TCCGCAC7050.014.19114264
GTATAGA15250.013.5738751
TATACAG20500.013.4635185
CTAATAC14800.013.0541543
TAGGGGG3204.1853673E-712.9375994
ATACAGC13650.012.8902096
CGCACCT6750.012.77746566
GTATATA31400.012.5255741
ACTATGC13400.012.3579068
CCTACAC6150.012.3415583
ACTATAC14900.012.2719073
CATATAC22050.012.2041753
GTCTAGG7450.012.0403611
CTAGGAC4652.062734E-911.8710593
TCTATAC14900.011.8088163
Produced by FastQC (version 0.11.2)