FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514692
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCG200.007357351551.74890524
GCGTCAG1052.3261236E-829.5708054
GCAGCGT1155.6223143E-827.0020541
CGTCAGA1208.500683E-825.8744535
ATAGGGG1052.2779852E-522.9995143
ACGGTGT1704.425601E-922.323068
GTGTAGT1103.2604785E-521.9562151
CAGCGTC1455.282327E-721.4133422
AGCGTCA1551.0024614E-620.0318363
ACAGCGC1056.017648E-419.713878
CTTAGAC1409.3286435E-619.713873
CCTGAGC900.005730904619.1662628
TATACGG1954.581907E-717.6919335
TACGGTG1653.8356455E-516.726927
CGGTGTT2151.2721066E-616.0461739
ACACCGT1300.002519529315.9227396
GTATTAG2201.6149916E-615.6830111
TTAGACC1554.5188013E-415.5803174
CTCCTAA1350.003238152515.33451
GATGTGT2051.4399753E-515.14602210
Produced by FastQC (version 0.11.2)