FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263341
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG3601.8189894E-1216.29290830
ACCCGCG1958.983554E-615.9242933
TACACTG6200.015.5804565
CTAATAC10650.015.5491073
GTATAGA8900.014.7304841
TCCGCAC5250.014.45664764
CGATCAC3851.03682396E-1014.33780524
TAATACT15950.013.8430914
ACCGTAC16700.013.84084369
ACCGAGC2751.0787662E-613.7998328
GTATTAG9850.013.6600331
CTAGGAC4052.3283064E-1013.6294643
GTATATG11400.013.6185571
CGTACAC3801.3023964E-913.6182553
GTATAGG6250.013.2481321
CGCACCT4951.8189894E-1213.2419766
CACCTAG5250.013.14327728
CCTAATA10650.012.9575882
AGACTAG4801.8189894E-1112.9373437
TATACAG10950.012.9176515
Produced by FastQC (version 0.11.2)