FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470397
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9350.016.2360171
ACCGTGC3101.8169885E-814.4678018
TACACTG7650.013.530295
CTAGGAC4102.8194336E-1013.4642883
TACACAG6300.013.1437095
CACGAGC4204.129106E-1013.14237969
TATACAG14900.012.9672835
TGGGGGG4551.07320375E-1012.8909465
CTAGGAG2701.3514393E-512.7786063
GTATAGG7300.012.7608431
CTAGACT3255.046968E-712.7392884
GTATATA25300.012.6823831
CCGCGCG2204.4406412E-412.5449989
TATACGG7900.012.2286415
CCGTGCT3408.726911E-712.1760289
TATAGGA9400.012.1127322
CAGTCCG2100.004465257711.4995819
TAGGACC4551.895387E-811.3743644
GTATAGA11850.011.3549371
TCTATAC10150.011.2174763
Produced by FastQC (version 0.11.2)