FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926226
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA6750.015.8453631
CTATGCA6550.015.8008019
GTATTAG6650.015.5648121
ACTATGC6950.015.3885828
GTATATA16150.015.3816971
GTCCTAC2302.574141E-615.000871
CCTAGCG1901.2819923E-414.5267830
TAGACTA3152.240995E-814.238555
ACTGTGC3951.5643309E-1013.9747678
CTAACCG1500.006532929413.7993679
CTAATAC8550.013.31621553
TACTATG7550.013.2517367
TACACCG3406.103255E-813.1915995
ATACAGT8150.013.1227796
TCTATAC8700.013.0866263
TAGGTAG4002.8012437E-912.9379145
TATATAG11500.012.9004135
CCTAATA7850.012.7459622
CCTACGC1900.00210976312.71027250
GGACAGT4104.0490704E-912.6220276
Produced by FastQC (version 0.11.2)