FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGGAAGGGGTGTGTCCTGTGGACCATTTTAAGTATTTTGGCTTTTACCCTCAAAAGAGGAAGCCGTGCCGGG30.22091310751104565No Hit
GGTCTAACATCCATGGCTTGTAGTTCGGTTCTAGGAATAAGGGGATTCAAACAAAATTAGGAGAGCTATATCCTG30.22091310751104565No Hit
AATATAAACTTGAAGTCAGGTAGCCGGGAAATTTTGCAAAAACAAAACAACCTATATTTTGAGGCTTGTCTTCTT20.14727540500736377No Hit
CACTTGGTGTGTGCTGGGGCTGAGATTTCGTGCAGAGGCCATTTGTGGAGTTGAACGCCAGTTTTTATGCCCTGT20.14727540500736377No Hit
AATCACCTGGGCAGCTTTTGAAAGAAATATTGTTTTCCTGGCTCCATCCCTGAACTATTAAATTAAAAATTATTG20.14727540500736377No Hit
CTCAAACCCTGCAACTCCTTTATCAACTAAGTTTACGTAATACTCTAAATCCTTTGTTGTCATTTCAACAACATT20.14727540500736377No Hit
GTGTACCAATAATAATAAGCATATTCTAAGCCTCTTCCCTGAGGATAAGAGAGCATTCTTAATTATGTCATTGTG20.14727540500736377No Hit
CCATGTACGTGACAAAACCCCAACTGAACGCAAACCAAGTTACCACGCATAGCACCAACGGCGCGAGGAGGAAGG20.14727540500736377No Hit
TTTTGAGATGGAGTCATCACCCAGGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAGCCTCTGCTTCCCAGG20.14727540500736377No Hit
CTCATATTCAGCACGAACGGGGCGACGACACCAGTATTGATTACCTGGAGGGAGGCCGTCGGGTTCTTCGCCTTG20.14727540500736377No Hit
TGGCAGGGGATAAAGAGAGGAAAAAGAAAAGACAAATTGAGTTAGCTTCATGCTATCTTTATTGGGTCTTGTTTG20.14727540500736377No Hit
ATCATCTTTCAGATGAGTTGTAATTTTAACGGAACTAAACTAAAAGCAGAGAATTGCATTTTTGTTTATTCTGAA20.14727540500736377No Hit
CATATTATATCCCTAATAGTATAGAACTCAACATTCACATTTTATAAAAAGTGAATTGCAATGGGCCTTATCAAT20.14727540500736377No Hit
GCCATCTCCTCCGGCTTTTTCTTACCCATCGCGCGACGCAGCATATCCTGTCTCTTATACACATCTCCGAGCCCA20.14727540500736377No Hit
ACCTATGAGAATTGCCTGGAAGTAAGCCCAGGACAACACACAGTAGGTAACTCAGAGTAAAGCCATTACAGGGAG20.14727540500736377No Hit
ATACCTAGTTTACTGAGAGTTTTTAGTATGAAGGGGTGTTGAATTTTATCAAAGACCTTTTCTGCATCTATTGAG20.14727540500736377No Hit
AAATCTCAGAAATCACCACTAAAGAACTTATTTGTGTAACCAAACACCCCTTTTCCCCCAAAAAACTATTGCTGT20.14727540500736377No Hit
CTCCTCAATTATGCAGTCTGGGTGAGTATGCCATCTATTTCTTGCAGGACCCAGGGCTGGTACAGTTTCTGTCTC20.14727540500736377No Hit
GTCTAGGACCGCCTTCATATTCTTTTTGACGTGGTCGTCGATCACACGCGGGCCAGACACGTAGTCGCCATACTC20.14727540500736377No Hit
GTACTTATGGTCCCTTTTAAGTGTAGGGCATGGTGCTGGGTTTAGGCATGGGGATGGTCTCAGGAGACTTTGCTG20.14727540500736377No Hit
GGCTGAAAATTCCAAAAACCAGAATGCCTCTTCTCCAAAGATTGCAACTCCTCGCCAGCAAGGGAACAAAACCTG20.14727540500736377No Hit
CTTGGAGACTCAGTACGGCACCACCACCATTGATGACGTCGTTGTTTCCAAGATCGCCGGCATTGCAGCCCGCGA20.14727540500736377No Hit
CCTTTATAGTTATTTTATTTATTAAAGAAAACATTTATTATAGGTTTAGGGATCATGTGAAGGTTTGTTATATCT20.14727540500736377No Hit
TCTATGAAGAATGTCTTTTGTAATTTAACATGGATTGCACTGTTTCTGTAGATCACATTGGGTGATATAGATATT20.14727540500736377No Hit
AAGTCATTGTTTATAGAAAGGAAGATAAAAACCACACAGGCAAAAAAGGTGGGGAATCAAACCTTTTTTAATACT20.14727540500736377No Hit
ATACTTAAGGAAGTATTAAGAGGTAGAAAGACATGATAAAGGTAACTGACTCAGAGAAGTGATATGTACTAAAAA20.14727540500736377No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG20.14727540500736377TruSeq Adapter, Index 11 (96% over 28bp)
TTCGATGATTCCATTCGATTCTATTCGATGATGATTGCATTCGGGTCCATGGATTATTCCATTCCATTCCATTAG20.14727540500736377No Hit
GTGCAGCGGCCCGAAGCTGTCCGGGCGGCCCTTGAAGCCGTTGAGCAGCGCCAGGGTGCTGGCCGTGGCCTCCTG20.14727540500736377No Hit
GCACACGCCTGTAGTCCCAGCTACTTGGGGGGCTGAGGCAGGACAATCATTTGAACCCGGCAGGTGGAGGTTGCA20.14727540500736377No Hit
AATCAATGTCATTGTTCTTGTAATGGTTCAGCAAGGAGAGAGACTTGCCTGTTAAGTTAGTGCCTTGAAACAAGT20.14727540500736377No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)