FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468974
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT4050.016.18474466
TCCGCAC4200.015.60671764
CTAATAC13350.015.5051943
CCGCACC4250.015.42310865
ACTATGC10100.015.3708688
CCCGTGC2252.050605E-615.3329158
CTCCTAG2959.44965E-915.2032821
GTATATA27900.014.5912951
TATACGG8450.014.2895515
GTATTAG11500.014.09991
CCTAATA13450.013.8509232
CCGTGCA3506.008122E-913.79962359
TAGACTG5900.013.4487855
GTATAGA13350.013.1796821
GCCCGAC1850.001726719413.05369811
CTGGGCA5300.013.0185139
TCTAGAC5850.012.9740053
GCGCACT2952.3757184E-612.86405511
CGTGCTA3002.8681789E-612.64965410
CTAGGAC3601.2889541E-712.45799353
Produced by FastQC (version 0.11.2)