FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316709
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCG400.00259275134.5020523
GTGTTCG450.004616342530.66849339
TCCGCAC450.004619910430.6636564
GCGTCAG1005.002694E-727.5972864
CCGCACC500.00773030927.59728665
CAGCGTC1057.6606557E-726.2831292
CGTCAGA1057.6606557E-726.2831295
GCAGCGT1151.6926278E-623.9976391
GTCTATA1052.2757647E-522.9977381
AGCGTCA1253.4929526E-622.0778273
GAGACAG1304.900725E-621.23203321
ACTATGC1004.328132E-420.6979648
AACCCTG1004.328132E-420.6979645
GATGTGT1356.803477E-620.44243210
GGCTGTC850.004116043420.29212267
TAGACCC900.00572869619.1647824
ACTATGT1108.224183E-418.816338
GTCAGAT1501.6890455E-518.3981886
TGGATCC950.00781956618.15897647
TATACCC950.00782669118.1561094
Produced by FastQC (version 0.11.2)