FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3907893
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG4750.014.52636054
TATACGG12750.012.4470985
GTATAAT24750.012.2670191
CTAATAC17050.012.1408013
GTATTAG15600.011.9426511
TATTAGG15950.011.8965892
TAATACT28950.011.7979644
GTATTAA21150.011.7450181
GACTAAG36950.011.29744669
TACACCG7550.010.9669235
AGGCGAA20850.010.75515569
TAGGTAG9000.010.7333675
ACTAAGG34100.010.72409969
GTATAGA14200.010.6904481
AGGGGGG7101.8189894E-1210.6901745
CTAAGGC27050.010.20309869
AAGGCGA23700.010.18964568
TACTATG18350.010.152367
CTTACAC9900.010.1060923
ACTATGC15750.010.0759648
Produced by FastQC (version 0.11.2)