FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112210
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC6550.015.27416964
CCGCACC6700.013.90240265
CTATGCA10100.013.3212249
ACTATGC12400.013.0760658
CGCACCT6100.013.00765166
TAGGACC5205.456968E-1212.6054434
CTAATAC14350.012.5014193
CCTAATA14650.012.4809062
GTATATA25200.012.4582121
GTATTAG10950.012.2875511
TAATACT22400.012.1670964
TGGGGGG5401.0913936E-1112.1385765
TATAATA34600.011.6658832
ATAGGAC8050.011.571133
TATATAC30350.011.4807583
ACTAAGG27350.010.97394669
AGACTAA37850.010.93745469
ATACAGG10750.010.91134556
TATACTG16850.010.8513515
GACTAAG27350.010.84780968
Produced by FastQC (version 0.11.2)