FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341759
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC14450.015.27979169
GGCGAAT23150.015.20037869
CACGAGC6100.014.13889469
TAGACAG10050.014.0745995
CTAGACA8650.013.9595114
ACTAAGG68400.013.5675569
AGGCGAA30400.013.1636668
CCCCTAG2901.9600884E-613.0867691
GACTAAG79450.012.85295769
GTATTAG11600.012.7893421
GTCCTAG4104.0508894E-912.6224941
TCTAGAC8600.012.4360243
TAAGGCG44150.012.18987269
CTAAGGC54500.012.15374169
AAGGCGA36200.011.72200169
CTAGACT5602.3646862E-1111.7053344
CACCCCG2752.2699227E-411.29038057
AGACTAA101000.011.03281669
TACTATA14700.011.0307562
TAGGTAG6603.6379788E-1210.9772515
Produced by FastQC (version 0.11.2)