FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837975
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG11750.018.2038565
TACGGTG9700.017.4274527
GTATTAG11100.017.0954231
CCGCACC4900.015.48918365
ACGGTGT10150.015.2952318
CATATAC23250.014.5418733
TATTAGG13050.014.2763022
TAGGTAG7500.014.2596875
ATACGGT12400.014.1892046
GTATAGG9600.014.0163041
GGTAGTC7300.013.7051787
GTATAGA15850.013.4959221
CGCACCT5400.013.41613666
TCCGCAC5150.013.39752864
TATACTG20100.013.3877655
TATACAG18300.013.3849525
CCCTGCG2609.172769E-613.2687069
TCATATA27700.012.9534692
ACTATGC11800.012.8641248
GAGCCCC3551.0744952E-712.63330369
Produced by FastQC (version 0.11.2)