FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725807
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3100.020.03184566
TCCGCAC3750.018.3996264
CTAGGAC3257.2759576E-1216.9847583
GTATTAG8050.016.2863241
ACGGTGT6650.015.5635898
TATACGG8000.015.525135
CTTAGGG1808.088545E-515.3334633
CCGCACC4300.015.24387265
TACACCG3857.2759576E-1215.2338955
CTAATAC9300.015.2098063
CTATGCA7800.015.0381519
TAGGTAG5300.014.9718245
TAGTCAG4151.8189894E-1214.9635489
CATATAC13200.014.8978533
TAATACT13900.014.3958044
AGGTAGT7050.014.1916086
AGCGGGG1951.599314E-414.15355450
TATACAG11800.014.0340165
GACTAAG13100.013.95772769
TACGGTG6450.013.906697
Produced by FastQC (version 0.11.2)