FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4729977
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG17050.015.58072955
TAGGTAG11850.015.1393225
ACTATGC19100.014.6308158
TAGACAG14500.013.0862775
TATACAG28400.012.9983095
GTATAGG14350.012.7426331
TAGATAG16950.012.6195365
CTAATAC24150.012.571633
TAGTCAG10500.012.4852529
TACACAG16450.012.3739275
CGCACCT7550.012.33729366
GTATTAG21600.012.29906751
TATAGGG12800.012.1292272
CCTATAC13500.012.0113033
TCCGCAC7800.011.94186664
CATATAC35600.011.6293993
CTTACAC12200.011.5944473
CTATGCA17300.011.5660479
AGACAGC14650.011.539316
CCGCACC8450.011.4315365
Produced by FastQC (version 0.11.2)