FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n01_minidip01_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n01_minidip01_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367290
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTG400.002594045134.59
GAGTCCG450.00461863930.66666644
GGCGGCA450.00461863930.66666611
ATGTCCG500.00772817927.69
GGTCTCG500.00772817927.615
AGAGCGC500.00772817927.68
CAGCGTC1101.1492084E-625.090912
GCAGCGT1101.1492084E-625.090911
ACGCATA700.001332120224.64285911
CCCTACA750.00199045623.02
AGCGTCA1253.4924888E-622.083
GAGCGCA953.0601284E-421.7894749
GCGTCAG1356.8025583E-620.4444454
AGCGGTT850.00411493220.29411742
TCTAGGG900.005727146819.1666663
CCTACAT1108.2221575E-418.8181823
ATCACAC950.0078245718.15789629
GACCAGT950.0078245718.15789632
TATACTG2851.6370905E-1118.1578965
CCTTGCG950.0078245718.1578969
Produced by FastQC (version 0.11.2)