FastQCFastQC Report
Wed 19 Aug 2020
HJNGMDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNGMDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152910812
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG85500450.3971388388382528No Hit
GCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGAG21992920.1021543478597199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT97288050.079.336471
AGCAGTG104760650.073.639292
CGCTCGA3279800.071.749216
GGCTCGA5361300.069.231626
CGGCTCG1171800.067.4384845
CTCGATG17269250.066.412568
GCTCGAT17742000.064.585067
GCAGTGG124785350.061.8297163
CAGTGGT139607500.055.2682274
GGGCTCG2433900.052.6493385
CCGCTCG949600.050.5666775
AGTGGTA155471850.049.6957975
GCGCTCG1651800.046.289995
TGGCTCG2041250.045.196915
TCGCTCG1456100.042.754515
GTGGTAT182349900.042.433986
GAGCTCG1586450.041.7275165
AGCTCGA5520350.041.5620546
GTATCAA191350850.040.438739
AGGCTCG1843350.040.393945
Produced by FastQC (version 0.11.5)