FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n02_4_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n02_4_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235473
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3837516.296985216988784No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGA400.00260204434.4732362
GTACTAA450.004623234330.6559011
TAACACA500.007703825427.61378362
TATATAG700.001335076824.6289715
TAAGCAT850.00412404620.2826826
GAGGCAA850.00412404620.2826825
TATTAGG850.00412912420.2783762
GAATGAG1108.241347E-418.8075776
CTGCACC950.007793948518.16696244
AGTGTAG2251.0956137E-716.86791269
TGTCCAA1250.001938984316.55418810
ACGAGTG4450.015.50676569
GAGTGTA2503.6298297E-715.18112368
GACGAGT5000.013.80102168
ATAATAC1500.006542066613.7892953
ACATATG1550.00812697613.3444793
CGAGTGT3406.0110324E-813.19215269
GTATTTA1600.00999450412.9329581
TATAAAG2000.003103427412.0656332
CGACGAG5853.6379788E-1211.79574467
Produced by FastQC (version 0.11.2)