FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n02_3_cut16h-2000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n02_3_cut16h-2000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225600
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3784316.774379432624116No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17700.7845744680851063No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16350.7247340425531915No Hit
CTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGG8340.3696808510638298Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCC200.007345795651.75998364
CGCATGC200.007358696351.73702622
GTTGGAC200.00736515351.725553
GTCACAC200.00737161451.7140851
AAGGGGG1900.047.21963565
TGGTCGC1900.043.5970341
ACCGTCT404.9965725E-543.114198
GTGGTCG2100.042.7320140
GGTCGCC1800.042.18416242
GCCGTAT1800.042.18416246
GGTGGTC2050.042.07195739
CGCCGTA1750.041.41717545
CCGTATC1850.041.03494347
CGTATCA1800.040.24883748
AAAGGGG2350.038.17757864
CGGTGGT2400.037.3739238
AGGGGGG2500.037.2671966
GTCGCCG2050.037.03975743
CTCGGTG2600.034.51431736
TCGGTGG2800.034.50665737
Produced by FastQC (version 0.11.2)