FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n02_2_cut16h-2000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n02_2_cut16h-2000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358866
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4928713.734095734898263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACC200.007349871651.75899542
GGCCCGT200.007374227551.7157176
CGTGTCG350.001347274939.42442712
AGGTGGG658.671901E-426.5245767
ATAGGCT953.076576E-421.7720014
ACCAAGC950.00784786918.1483928
ATAAGGC1250.001947523116.5467223
CGAGTGT6000.016.102869
ATTATAC2001.1462756E-515.5125533
GACGATT1400.004126353714.78416127
AGTGTAG4251.8189894E-1214.61430469
AGATCTC1450.005199623314.27834469
GTGTAGA3454.840331E-914.00243469
ATTGTCC1500.006539928713.7927798
CTATTAG1500.0065459713.7908571
TAGAAGT1500.006552016413.7889364
TTGGATG1550.00813928913.34413054
ACGAGTG7450.012.96869768
CTGCCGC1600.00997324512.93974969
TATAACA2153.6678702E-412.8269172
Produced by FastQC (version 0.11.2)