FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n02_1_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n02_1_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508706
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6064411.921227585285017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCC200.00735627251.75073661
CTCTGGG350.00134571439.43688642
GTCGCCG754.0057785E-836.80776243
GTGGTCG851.2039527E-732.47743640
CGGTGGT1001.4464604E-831.05044638
TCGGTGG1052.3230314E-829.57475737
GGTCGCC1004.9992195E-727.60582242
CCTCGAC500.00773039227.60039511
TAGAGGT658.65965E-426.5336234
GGTGGTC1151.6928843E-624.00270339
TCTCGGT2103.6379788E-1223.00258869
GGATAGT750.001992477622.99806616
ATCTCGG2155.456968E-1222.46764468
TGGTCGC1253.4904733E-622.08465841
TTAGACC800.00290030221.5585674
CTCGGTG1356.804403E-620.44674736
GATCTCG2556.730261E-1118.9433167
CGCCGTA1108.216444E-418.8221545
TATACAG1702.431092E-618.2613755
GTGTAGA6650.018.15993969
Produced by FastQC (version 0.11.2)