FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n01_4_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n01_4_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235473
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3543115.0467357191695No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG10970.4658708217077967RNA PCR Primer, Index 23 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGG200.007344648751.7627453
CGCCTCG200.00735699951.7407614
AGGGGGG2350.048.458745
CGTATGC1850.046.613341
TATGCCG1850.046.613343
CAGGGGG451.8519113E-646.0113334
ACAGTGT306.306729E-445.991798
GAGGATC1750.045.33476633
TAGGGGG1150.045.011084
ATGCCGT2000.044.8419944
GTATGCC1950.044.22287442
CTCGTAT1950.042.4539639
TCGTATG1950.042.4539640
CTGGGGG758.6401997E-1041.41024
TCGGGGG503.823274E-641.4101944
CGTCTTC2000.041.3926148
TGCCGTC2100.041.06409545
TCTCGTA1950.040.68504338
ATCTCGT2150.040.10911637
TTGGGGG1550.040.0743874
Produced by FastQC (version 0.11.2)