FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n01_3_cut16h-2000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n01_3_cut16h-2000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225600
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2485511.017287234042552No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG142946.335992907801419RNA PCR Primer, Index 39 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG13000.576241134751773Illumina PCR Primer Index 11 (96% over 29bp)
GTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG6870.3045212765957447RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG3890.1724290780141844TruSeq Adapter, Index 11 (96% over 28bp)
CTTAATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG2730.12101063829787234RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG18150.065.2427869
TAGGGGG353.26987E-759.156924
GTATGCC20300.058.9607842
GCCGTCT20300.058.79086346
TCGTATG20200.058.56963740
CTCGTAT20200.058.3988839
TATGCCG20500.058.38555543
CGTATGC20550.058.243541
TGCCGTC20650.058.12848745
TCTCGTA19350.058.1120838
ATGCCGT20650.057.9614544
AAAGGGG20500.057.93214468
CCGTCTT20650.057.62737747
CGTCTTC20800.057.54345748
CATCTCG19400.057.42891336
TGCTTGA21100.057.0775555
CTGCTTG21000.057.02069554
ATCTCGT19600.057.0188937
TCTGCTT21050.056.88525453
TTCTGCT21000.056.6794852
Produced by FastQC (version 0.11.2)