FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n01_2_cut16h-2000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n01_2_cut16h-2000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358866
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4945613.781188521620882No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG8620.2402010778396393TruSeq Adapter, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1500.055.19420645
GATCTCG1500.052.89444736
GCCGTCT1600.051.74456446
TAGGGGG950.050.8367654
TATGCCG1650.050.17654443
AGGGGGG1850.048.4813965
CCATAGG306.278615E-446.0400731
CCGTCTT1850.044.75205647
GTATGCC1850.044.75205642
TCGTATG1950.042.4570840
CGTATGC2050.040.38600541
AAGGGGG2000.039.70402569
CTCGTAT2100.039.4244339
ATGCCGT2100.039.4244344
TCTCGTA2200.037.63241238
TGATCTC2200.036.06439635
ATCTCGT2300.034.4963837
ATGGGGG1400.034.4963764
TACGTCA400.002595302634.49637616
CGTCTTC2350.033.76241348
Produced by FastQC (version 0.11.2)